The stock from the Scripps Research Institute in San Diego and his research has been mostly focused on cognitive aspects of addiction so he did his bachelor’s degree at University of Texas where he got a degree in psychology he then got his PhD at UT El Paso where he worked with Laura O’Dell and Tony Shipman Berg at the National.
Institute of drug abuse studying mechanisms of nicotine dependence but Louis’s I did nicotine was that for him and he needs something a little more complicated so.
He went to the Scripps Research Institute where he worked with Larry Parsons Marissa Roberto studying mechanisms of alcohol dependence specifically looking at the interactions of the stress system with cortical function Lewis is well published she has over 19 publications including prestigious journals like Biological Psychiatry J neuroscience and proceedings the National Academy of Sciences he has been well funded he has both a f31 he was on a t32 training grant and currently.
Hold the k99 grant from the national institute of alcoholism and to supplement a lot of his psychology.
Background he decided to go all-in on learning at molecular biology and is currently doing his studies with John Yates who is a world expert in the.
Area of proteomics to try and identify new targets that are driving alcoholism again he has so much in his background that I could go on forever but what I want to do is allow Louise to talk for himself and tell us about some of the work that he’s been doing with endocannabinoids say going in stress so I leave the floor to Louise good luck thank you.
So much for that nice introduction okay so thank thank you for that wonderful introduction man so you know thank you so much everyone for being here today for inviting.
Me over this is indeed an exciting time in.
My career in terms of conceptualizing the next phase of my academic career and I’m happy to have the opportunity to present this vision for you today I obtained my doctorate at the University of Texas at El Paso under the guidance of dr.
Laura O’Dell and so in Laura’s lab I learned how to.
Apply behavioral and neural chemical tools for the understanding of the effects of chronic nicotine exposure in the adolescent brain for my postdoc I joined the you know the laboratory of dr. Larry Parsons to start to apply molecular strategies using mass spectrometry to in my work so that I could study a variety of different neurotransmitters and explore the relevance to behaviors that are related to the dark side of addiction unfortunately Larry passed away during this during my.
Continue these efforts with Marissa Roberto and then received a k99 award to expand my skill set into untargeted proteomics and John Yates is lab and so I’m here to talk to you today about some of the work that derived from a collaboration between the Roberts of Parsons laboratory and an international laboratory at the University of Comedy you know in Italy.
With Roberto Chico Chopra and so my goal here is to emphasize some of the some of the ways that we probed endocannabinoid function and explore its relation to stress and anxiety and you know part of this will be a focus of my ongoing research program so the title of my talk is impaired endocannabinoid signaling and stress and addiction so my my understanding is that I’m speaking to.
A diverse group of scientists and and as such I wanted to begin by.
Touching briefly on a couple of basic principles in drug.
Addiction particularly related to the dysregulation and motivation in reinforcement oh I think of these introductory slides as a moment but I’m not teaching hat and and describe where we’re at in terms of describing the philosophy of addiction and the mechanisms that we think are important for driving this process and then I’ll go ahead and move on to a discussion of.
The endogenous cannabinoids system defined here as an important anti stress mechanism in the brain and then I’ll dive into some.
Of my research investigating the role of endocannabinoids.
And modulating withdrawal related anxiety drug suppose into some of.
The data that I collected from a genetic model exhibiting at comorbid phenotype of affective disruptions as well as alcohol preference and then finally I’ll end up on some of the future directions of this work sort of building off of this premise of stress anti stress and interactions the neuro proteome and further.
Implications on cognitive function and so.
Addiction continues to be a major problem in the United.
States and although we have come to understand addiction in our own way we should know that it means something very specific in the clinic and.
The same substance use disorder is comprised of multiple symptoms that basically generalized to the compulsive and recurrent nature of drug abuse despite the harm that it inflicts in individuals and so dependence and withdrawal have informed an interesting corollary for the study of the.
Diction due to the long term consequences of abuse that are often described in opposition to the effects of the drug themselves and so this suggests that whatever compels an individual to abuse drugs may change over time in it difficult to apply a comprehensive.
Strategy to resolve these types of issues and so the symptoms of addiction have been conceptualized indifference of theoretical models so shown here is the representation of addiction.
In three different stages preoccupation anticipation and binge.
Intoxication and withdrawal negative after and so basically excess drug use mobilizes biological resources that promote negative behavioral responses particularly when the drug is.
Not on board and this leads to further episodes of drug craving drug drug binging and so persistent question in the field is what are the processes that drive the transition from casual used to dependence effectively leading to this vicious.
Cycle we know the sped the stages that ultimately promote the loss of control of drug use and susceptibility to relapse and so you.
Aspects of addiction that that I’ll focus on a little bit.
More is this sorry is this notion of what all as it remains kind of this.
Pivotal reason why why people find it difficult to maintain their sobriety and so whereas like conventional therapeutics have been successfully employed during the early phases of withdrawal and we’ve been able to control the physical.
Symptoms that manifests therein negative effect can actually persist for a longer.
Period of time and and therefore present a major hurdle for recovery and so to gain a better understanding of these processes scientists may look to study changes and motivate a behavior and so motivation can be broadly defined as a desire or willingness to engage in an action so Clarke whole introduced this interesting idea of the concept of motivation as a drive state and so that is behavior being an.
Expression aimed at fulfilling the basic biological Drive and so an example of the as hunger in which that motivates an organism to forage for food in order to reduce.
His drive state so we now know that that motivated behaviors in quite this.
Simple but it imported an important framework for hypothesizing the aspects of addiction and so more generally addiction seems to play on the notion of basic motivational systems that orient an organism towards stimuli that induce rewarding responses whereas aversive.
Stimuli have the capability of inducing aversion and have a you know people end up trying to move away from.
These stressors or it may result in some other compensatory action and to mitigate this consequence and so.
From a psychological perspective these concepts overlap with operational definitions of reinforcement and so initially non-dependent drug use is motivated by positive reinforcement and this is the incidence where the presentation of a stimulus.
Or drug increases the likelihood of responding and so drug reworking induce sensations such as a buzz of pleasurable sensation or euphoria and other effects that are attributed to feeling good however as one becomes drug experience there is a tolerance to these initial responses leading to the.
Loss and the drug reward component and so in theory you know this should be enough to make make it easier for people to stop taking drugs and this is.
Probably enough of a reason for them to stop however from you know the this may.
Also prompt escalated intake and this may move an individual towards dependence injuries drug taking and we have studied this phenomenon in our department in terms of negative reinforcement where the removal.
Of an aversive stimulus or negative emotion State increases the likelihood of responding and so in this sense drug use is thought to be ingrained with the strategy of relieving negative effect like stress and anxiety depression ballets you know even aim that emerges during withdrawal and so an important.
This research is what are the neurological substrates and modulate negative effects and so with the better understanding.
Of how these mechanisms work we might be able to better identify druggable targets for further study in clinical therapeutics so withdrawal is especially important in the study of alcoholism.
Given the severity of symptoms symptoms that are expressed in the clinic and so for example highly dependent individuals often experience delirium tremens which is a condition that is associated with a temporary increase in.